RT journal article
T1 Ex situ and in situ functionalized Yb/Fe nanoparticles obtained by scanning pulsed laser ablation in liquids: A route to obtain biofunctionalized multiplatform contrast agents for MRI and CT imaging
A1 Félix Ruiz, Eduardo José
A1 Manuel Delgado, José Manuel
A1 Fernández Ponce, Cecilia Matilde
A1 Yeste Siguenza, María del Pilar
A1 Lahoz, Ruth
A1 Rodríguez, M.A.
A1 Abasolo, Ibane
A1 Llaguno-Munive, Monserrat
A1 Fernández Cisnal, R.
A1 García Villar, Cristina
A1 García Cózar, Francisco José
A1 Litrán Ramos, Rocío
A1 Bomati Miguel, Óscar
A2 BiomedicinaBiotecnología y Salud Pública
A2 Física de la Materia Condensada
K1 Laser ablation in liquids
K1 Functionalization
K1 Biocompatible ytterbium/iron nanoparticles
K1 Gluthathione
AB Two distinct strategies were used to improve the colloidal properties of hybrid Yb/Fe oxide NPs previously prepared by the pulsed liquid laser ablation process for use as a contrast agent in medical imaging. First, an exhaustive optimization process of the laser ablation synthesis parameters was carried out to reduce the hydrodynamic diameters of the Yb/Fe NPs. The hydrodynamic size was successfully reduced to <200 nm, thereby decreasing the polydispertivity index. Second, ex situ and in situ functionalization processes using glutathione, cysteamine, or polyethylenimine as capping agents have been developed to increase their colloidal stability at physiological pH values. Transmissionelectron microscopy, dynamic light scattering, Z-potential measurements, and Fourier Transform Infrared spectroscopy were used to examine the structure, morphology, colloidal and surface properties of Yb/Fe NPs. Colloidal stability of the Yb/Fe NPs as well as the linkage mechanism of functionalization have been studied extensively. This last parameter provides a critical information for subsequently bioconjugations in biomedical applications. Additionally, the biocompatibility of the Yb/Fe NPs was evaluated by MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide) experiments. These results indicate more appropriate colloidal characteristics and higher biocompatibility for ex situ-functionalized Yb/Fe NPs, especially when the capping agent is glutathione. Additionally, these Yb/Fe NPs show good magnetic resonance imaging and X-ray computerized tomography imaging abilities, thereby indicating promising potential as dual contrast agents.
PB ELSEVIER
SN 0032-5910
YR 2023
FD 2023-06-14
LK http://hdl.handle.net/10498/31116
UL http://hdl.handle.net/10498/31116
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026