RT journal article
T1 Expression profile of microRNAs related with viral infectivity, inflammatory response, and immune activation in people living with HIV
A1 Cuesta Sancho, Sara
A1 Márquez Ruiz, Denisse
A1 Illanes Álvarez, Francisco
A1 Campaña Gómez, Irene
A1 Martín Aspas, Andrés
A1 Trujillo Soto, María Teresa
A1 Romero, Alberto
A1 Galán Sánchez, Fátima
A1 Rodríguez Iglesias, Manuel Antonio
A1 Márquez Coello, María de las Mercedes
A1 Girón González, José Antonio
A2 BiomedicinaBiotecnología y Salud Pública
A2 Medicina
K1 bacterial translocation
K1 Human Immunodeficiency (HIV)
K1 Intestinal Barrier Disruption (I-FABP)
K1 interleukin (IL)-6
K1 sCD163
K1 miRNAs
K1 people living with HIV
AB Objective: To evaluate the serum expression of microRNAs (miRNAs) with ability to modulate the human immunodeficiency (HIV) replication or inflammatory status in people living with HIV (PLWH). Methods: Forty healthy controls and two groups of PLWH were evaluated: (a) Group 1 (n = 30), patients with detectable viral load at inclusion, analyzed before receiving antiretroviral therapy (ART) and 12 months after initiating it; (b) Group 2 (n = 55), PLWH with prolonged undetectable viral load. Intestinal barrier disruption (I-FABP) and bacterial translocation (16S rDNA) markers, inflammatory markers such as interleukin (IL)-6 and sCD163, immune activation and expression of specific miRNAs were evaluated. Results: Serum concentrations of I-FABP, 16S rDNA, IL-6, sCD163 and activated T lymphocytes were increased in PLWH. Serum miR-34a was overexpressed at inclusion and remained elevated after ART. The expression of the remaining miRNAs that modulate HIV infectivity (miR-7, mir-29a, miR-150, and miR-223) was similar in PLWH and controls. Related to miRNAs implicated in inflammation (miR-21, miR-155, and miR-210), significant overexpression were observed in miR-21 and miR-210 levels in untreated PLWH, but levels were restored in those patients treated for a long period. Conclusion: A sustained overexpression of miR-34a was detected even after prolonged HIV controlled replication. miR-21 and miR-210 can be considered new markers of inflammation with high sensitivity to its modifications.
PB Frontiers Media
SN 1664-302X
YR 2023
FD 2023-03-02
LK http://hdl.handle.net/10498/31173
UL http://hdl.handle.net/10498/31173
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026