RT journal article
T1 Molecular Antioxidant Functions are Enhanced in Atlantic Bluefin Tuna (Thunnus Thynnus, L.) Larvae Fed Selenium-Enriched Rotifers Brachionus Rotundiformis
A1 Wischhusen, Pauline
A1 Betancor, Mónica B.
A1 Sprague, Matthew
A1 Ortega, Aurelio
A1 de la Gándara, Fernando
A1 Tocher, Douglas R.
A1 Mourente Cano, Gabriel
A2 Biología
K1 antioxidant enzymes
K1 bluefin tuna
K1 gene expression
K1 growth performance
K1 larvae
K1 rotifer enrichment
K1 Se-yeast
K1 selenoprotein
AB Selenium (Se) is an essential trace element for fish with more than 40 selenoproteins identified, many exhibiting antioxidant functions. This study investigated the effect of dietary Se supplementation on physiological parameters, selenoprotein and antioxidant enzyme gene expression in Atlantic bluefin tuna (ABT, Thunnus thynnus) larvae. First-feeding ABT larvae were divided into triplicate groups and fed rotifers Brachionus rotundiformis enriched with five different levels of Se (0, 3, 10, 30, and 100 µg Se·L−1) until 14 days after hatching. Both rotifers and ABT larvae effectively accumulated Se achieving maximum levels in the Se100 treatment (30.05 μg Se·g−1 and 194 ± 38 μg Se·g−1 dry mass, respectively). Larvae showed highest total length when fed Se3 rotifers, whereas flexion index was highest in larvae fed Se10. Selenium supplementation increased the gene expression of selenoproteins gpx1, msrb1, trxr2, selenom, selenop, and selenoe compared to the non-supplemented control (Se0), but only marginal differences were detected between supplementation levels. In contrast, expression of the antioxidant enzymes cat and sod1 were lowest in larvae fed Se100. To conclude, non-Se-enriched rotifers may be suboptimal for first feeding ABT larvae, which showed improved selenoprotein and antioxidant gene expression when fed a diet containing 4.42 μg Se·g−1 dry mass.
PB MDPI
SN 2076-3921
YR 2022
FD 2022-12-23
LK http://hdl.handle.net/10498/31399
UL http://hdl.handle.net/10498/31399
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026