RT journal article T1 Implementation of a Pilot-Scale Biotrickling Filtration Process for Biogas Desulfurization under Anoxic Conditions Using Agricultural Digestate as Trickling Liquid A1 Lenis, Alejandra A1 Ramírez Muñoz, Martín A1 González Cortés, José Joaquín A1 Ooms, Kristoffer A1 Pinnekamp, Johannes A2 Ingeniería Química y Tecnología de Alimentos K1 anoxic desulfurization K1 biotrickling filter K1 denitrification K1 hydrogen sulfide AB A pilot-scale biotrickling filter (BTF) was operated in counter-current flow mode under anoxic conditions, using diluted agricultural digestate as inoculum and as the recirculation medium for the nutrient source. The process was tested on-site at an agricultural fermentation plant, where real biogas was used. The pilot plant was therefore exposed to real process-related fluctuations. The purpose of this research was to attest the validity of the filtration process for use at an industrial-scale by operating the pilot plant under realistic conditions. Neither the use of agricultural digestate as trickling liquid and nor a BTF of this scale have previously been reported in the literature. The pilot plant was operated for 149 days. The highest inlet load was 8.5 gS-H2Sm−3h−1 with a corresponding removal efficiency of 99.2%. The pH remained between 7.5 and 4.6 without any regulation throughout the complete experimental phase. The analysis of the microbial community showed that both anaerobic and anoxic bacteria can adapt to the fluctuating operating conditions and coexist simultaneously, thus contributing to the robustness of the process. The operation of an anoxic BTF with agricultural digestate as the trickling liquid proved to be viable for industrial-scale use. PB MDPI SN 2306-5354 YR 2023 FD 2023-01-25 LK http://hdl.handle.net/10498/31450 UL http://hdl.handle.net/10498/31450 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 05-nov-2026