RT journal article
T1 Changes in Adhesion and the Expression of Adhesion Molecules in PBMCs after Aneurysmal Subarachnoid Hemorrhage: Relation to Cerebral Vasospasm
A1 Revilla González, Gonzalo
A1 Varela, Lourdes María
A1 Ruiz de Azua-López, Zaida
A1 Amaya Villar, Rosario
A1 Pezzotti, María Rosa
A1 Castro, María José
A1 Ureña, Juan
A1 González Montelongo, María del Carmen
A1 Castellano, Antonio
A2 BiomedicinaBiotecnología y Salud Pública
K1 Aneurysmal subarachnoid hemorrhage (aSAH)
K1 Peripheral blood mononuclear cells (PBMCs)
K1 Vasospasm (VSP)
K1 Adhesion to the endothelium
K1 Adhesion molecules
AB Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a current subject of research. We have analysed the changes in PBMCs of patients with aSAH and their interaction with the endothelium, focusing on their adhesion and the expression of adhesion molecules. Using an in vitro adhesion assay, we observed that the adhesion of PBMCs of patients with aSAH is increased. Flow cytometry analysis shows that monocytes increased significantly in patients, especially in those who developed vasospasm (VSP). In aSAH patients, the expression of CD162, CD49d, CD62L and CD11a in T lymphocytes and of CD62L in monocytes increased. However, the expression of CD162, CD43, and CD11a decreased in monocytes. Furthermore, monocytes from patients who developed arteriographic VSP had lower expression of CD62L. In conclusion, our results confirm that after aSAH, monocyte count and adhesion of PBMCs increase, especially in patients with VSP, and that the expression of several adhesion molecules is altered. These observations can help predict VSP and to improve the treatment of this pathology.
PB Springer
SN 1868-4483
YR 2024
FD 2024-02-23
LK http://hdl.handle.net/10498/31628
UL http://hdl.handle.net/10498/31628
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026