RT journal article
T1 Barcoding coffee grounds - Exploring pteropod gastropod biodiversity with dregs in collection jars
A1 Laibl, Christina Franziska
A1 Cervera Currado, Juan Lucas
A1 Morinière, Jérôme
A1 Schrödl, Michael
A2 Biología
K1 Mollusca
K1 Gastropoda
K1 plankton
K1 environmental DNA
K1 circumglobally
K1 pteropod diversity
K1 Malaspina expedition
AB Despite their cosmopolitan occurrence and massive plankton sampling during expeditions, the genetic diversity within Pteropoda Cuvier, 1804 is still largely unexplored. In this study we present a next-generation environmental barcoding approach to zooplankton bulk samples, which were collected during the circumglobal 2010 Malaspina expeditionto evaluate pteropod diversity. We introduce a technique that avoids destructive procedures and leaves material intact forfurther morphological investigations. We extracted DNA out of the dregs (organic material such as mucus or body parts)of 27 sample containers for molecular barcoding (average 100-260 bp of COI). We were able to identify 7128 operationaltaxonomic units corresponding to the species composition contained in the examined samples. Among them were threespecies of thecosome pteropods, Creseis acicula, Creseis virgula and Cavolinia inflexa, which are discussed with respectto their taxonomy and their geographic distribution. Unidentified gymnosomes were also present in our samples fromwarmer regions in oceanic waters of the southern Indian Ocean. To facilitate identification of species, it is beneficial tocreate a better database of pteropod COI barcodes. Furthermore, gathering environmental barcoding data on a broad globalscale will help to better understand species abundance and distribution of pteropods in the world’s oceans, and potentiallythose of other planktonic organisms.
PB CSIC Consejo Superior de Investigaciones Cientificas
SN 0214-8358
YR 2023
FD 2023
LK http://hdl.handle.net/10498/31742
UL http://hdl.handle.net/10498/31742
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 22-sep-2026