RT journal article
T1 Enhancing Efficiency of Enzymatic-Assisted Extraction Method for Evaluating Bioactive Compound Analysis in Mulberry: An Optimization Approach
A1 Tizón Alba, A.
A1 Aliaño González, María José
A1 Palma Lovillo, Miguel
A1 Fernández Barbero, Gerardo
A1 Carrera Fernández, Ceferino Adrián
A2 Química Analítica
K1 Mulberries
K1 Enzyme-assisted extraction
K1 Antioxidant activity
K1 Antimicrobial properties
K1 Box–Behnken optimization
K1 Plackett–Burman
AB The present investigation endeavors to optimize a method based on enzyme-assisted extraction for the efficient retrieval of bioactive compounds from mulberry, leveraging its notable health-promoting properties. A combined approach of Plackett–Burman design followed by Box–Behnken design was employed for determining the crucial extraction parameters and subsequently, refining the process. Optimal conditions consisted of heating 0.15 g of mulberry at 40 °C, using 15 mL of 70% EtOH as a solvent at pH 4, 38.46 enzyme units per g of sample, and shaking at 200 rpm. The optimum extraction time study revealed that 5 min of extraction was sufficient to reach the maximum concentration of the bioactive compound. The repeatability and intermediate precision assessment exhibited a coefficient of variation below 5%. Among the diverse mulberry varieties scrutinized, Morus nigra showed the highest anthocyanin content (27.90 ± 2.14 mg/100 g), while Morus rubra showed the highest concentration of phenolic compounds (121.10 ± 19.56 mg/100 g). Moreover, the extracted compounds showcased significant antioxidant and antimicrobial properties.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2073-4395
YR 2023
FD 2023
LK http://hdl.handle.net/10498/31822
UL http://hdl.handle.net/10498/31822
LA eng
NO Ministry of Universities of the Government of SpainUniversidad de Cádiz
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026