RT journal article
T1 Protection and Repair of the Nigrostriatal Pathway with Stem-Cell-Derived Carotid Body Glomus Cell Transplants in Chronic MPTP Parkinsonian Model
A1 Villadiego, Javier
A1 Muñoz Manchado, Ana Belén
A1 Sobrino, Verónica
A1 Bonilla Henao, Victoria
A1 Suárez Luna, Nela
A1 Ortega Sáenz, Patricia
A1 Pardal, Ricardo
A1 López Barneo, José
A1 Toledo Aral, Juan J.
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
K1 carotid body stem cells
K1 cell therapy
K1 neuroprotection
K1 neurorestoration
K1 Parkinson’s disease
AB Antiparkinsonian carotid body (CB) cell therapy has been proven to be effective in rodent and nonhuman primate models of Parkinson’s disease (PD), exerting trophic protection and restoration of the dopaminergic nigrostriatal pathway. These neurotrophic actions are mediated through the release of high levels of glial-cell-line-derived neurotrophic factor (GDNF) by the CB transplant. Pilot clinical trials have also shown that CB autotransplantation can improve motor symptoms in PD patients, although its effectiveness is affected by the scarcity of the grafted tissue. Here, we analyzed the antiparkinsonian efficacy of in vitro-expanded CB dopaminergic glomus cells. Intrastriatal xenografts of rat CB neurospheres were shown to protect nigral neurons from degeneration in a chronic MPTP mouse PD model. In addition, grafts performed at the end of the neurotoxic treatment resulted in the repair of striatal dopaminergic terminals through axonal sprouting. Interestingly, both neuroprotective and reparative effects induced by in vitro-expanded CB cells were similar to those previously reported by the use of CB transplants. This action could be explained because stem-cell-derived CB neurospheres produce similar amounts of GDNF compared to native CB tissue. This study provides the first evidence that in vitro-expanded CB cells could be a clinical option for cell therapy in PD.
PB MDPI
SN 1661-6596
YR 2023
FD 2023-03-14
LK http://hdl.handle.net/10498/31827
UL http://hdl.handle.net/10498/31827
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026