RT journal article
T1 Effects of Sesquiterpene Lactones on Primary Cilia Formation (Ciliogenesis)
A1 Murillo-Pineda, Marina
A1 Coto-Cid, Juan M.
A1 Romero, María
A1 García Zorrilla, Jesús
A1 Chinchilla Salcedo, Nuria
A1 Medina-Calzada, Zahara
A1 Varela Montoya, Rosa María
A1 Juárez-Soto, Álvaro
A1 Macías Domínguez, Francisco Antonio
A1 Reales Rodríguez, Elena
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Orgánica
K1 sesquiterpene lactones
K1 grosheimin
K1 costunolide
K1 primary cilia
K1 ciliogenesis
AB Sesquiterpene lactones (SLs), plant-derived metabolites with broad spectra of biological effects, including anti-tumor and anti-inflammatory, hold promise for drug development. Primary cilia, organelles extending from cell surfaces, are crucial for sensing and transducing extracellular signals essential for cell differentiation and proliferation. Their life cycle is linked to the cell cycle, as cilia assemble in non-dividing cells of G0/G1 phases and disassemble before entering mitosis. Abnormalities in both primary cilia (non-motile cilia) and motile cilia structure or function are associated with developmental disorders (ciliopathies), heart disease, and cancer. However, the impact of SLs on primary cilia remains unknown. This study evaluated the effects of selected SLs (grosheimin, costunolide, and three cyclocostunolides) on primary cilia biogenesis and stability in human retinal pigment epithelial (RPE) cells. Confocal fluorescence microscopy was employed to analyze the effects on primary cilia formation (ciliogenesis), primary cilia length, and stability. The effects on cell proliferation were evaluated by flow cytometry. All SLs disrupted primary cilia formation in the early stages of ciliogenesis, irrespective of starvation conditions or cytochalasin-D treatment, with no effect on cilia length or cell cycle progression. Interestingly, grosheimin stabilized and promoted primary cilia formation under cilia homeostasis and elongation treatment conditions. Thus, SLs have potential as novel drugs for ciliopathies and tumor treatment.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2072-6651
YR 2023
FD 2023
LK http://hdl.handle.net/10498/31907
UL http://hdl.handle.net/10498/31907
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026