RT journal article
T1 Production of (10S,11S)-(—)-epi-Pyriculol and Its HPLC Quantification in Liquid Cultures of Pyricularia grisea, a Potential Mycoherbicide for the Control of Buffelgrass (Cenchrus ciliaris)
A1 García Zorrilla, Jesús
A1 Masi, Marco
A1 Clement, Suzette
A1 Cimmino, Alessio
A1 Meyer, Susan
A2 Química Orgánica
K1 (10S,11S)-(—)-epi-pyriculol
K1 HPLC method
K1 qualitative and quantitative analysis
K1 phytochemistry
K1 natural products
AB (10S,11S)-(—)-epi-pyriculol is a phytotoxic metabolite produced by Pyricularia grisea, afungus identified as a foliar pathogen on the invasive weed species buffelgrass (Cenchrus ciliaris)in North America. The effective control of buffelgrass has not yet been achieved, and there is aneed to develop effective and green solutions. Herbicides based on natural products and the useof phytopathogenic organisms could provide the most suitable tools for the control of weeds suchas buffelgrass. Thus, one of the most relevant points to study about potential suitable phytotoxinssuch as (10S,11S)-(—)-epi-pyriculol is its production on a large scale, either by isolation from fungalfermentations or by synthesis. For these purposes, rapid and sensitive methods for the quantificationof (10S,11S)-(—)-epi-pyriculol in complex mixtures are required. In this study, a high-pressure liquidchromatography (HPLC) method for its quantification was developed and applied to organic extractsfrom twelve P. grisea isolates obtained from diseased buffelgrass leaves and grown in potato dextrosebroth (PDB) liquid cultures. The analysis proved that the production of (10S,11S)-(—)-epi-pyriculolis fungal-isolate dependent and strongly correlated with phytotoxic activity, shown by the P. griseaorganic extracts in a buffelgrass radicle elongation test. The HPLC method reported herein allowedus to select the best strain for the production of (10S,11S)-(—)-epi-pyriculol and could be useful forselecting the best cultural conditions for its mass production, providing a tool for the use of thispromising metabolite as a new bioherbicide for the control of buffelgrass.
PB MDPI
SN 2309-608X
YR 2023
FD 2023
LK http://hdl.handle.net/10498/32035
UL http://hdl.handle.net/10498/32035
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026