RT journal article
T1 Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain
A1 Olvera Collantes, Lucía
A1 Moares Fernández, Noelia del Carmen
A1 Fernández Cisnal, R.
A1 Muñoz Miranda, Juan Pedro
A1 González García, Pablo
A1 Gabucio López, Antonio
A1 Freyre Carrillo, Carolina
A1 Jordan-Chaves, Juan de Dios
A1 Trujillo-Soto, Teresa
A1 Rodriguez-Martinez, María P.
A1 Martin-Rubio, Maria I.
A1 Escuer, Eva
A1 Rodríguez Iglesias, Manuel Antonio
A1 Fernández Ponce, Cecilia Matilde
A1 García Cózar, Francisco José
A2 BiomedicinaBiotecnología y Salud Pública
K1 antibody response
K1 COVID-19
K1 IgG antibodies
K1 monitoring tool
K1 multiplex immunoassay
K1 SARS-CoV-2
AB There is still a long way ahead regarding the COVID-19 pandemic, since emerging waves remain a daunting challenge to the healthcare system. For this reason, the development of new preventive tools and therapeutic strategies to deal with the disease have been necessary, among which serological assays have played a key role in the control of COVID-19 outbreaks and vaccine development. Here, we have developed and evaluated an immunoassay capable of simultaneously detecting multiple IgG antibodies against different SARS-CoV-2 antigens through the use of Bio-PlexTM technology. Additionally, we have analyzed the antibody response in COVID-19 patients with different clinical profiles in Cadiz, Spain. The multiplex immunoassay presented is a high-throughput and robust immune response monitoring tool capable of concurrently detecting anti-S1, anti-NC and anti-RBD IgG antibodies in serum with a very high sensitivity (94.34–97.96%) and specificity (91.84–100%). Therefore, the immunoassay proposed herein may be a useful monitoring tool for individual humoral immunity against SARS-CoV-2, as well as for epidemiological surveillance. In addition, we show the values of antibodies against multiple SARS-CoV-2 antigens and their correlation with the different clinical profiles of unvaccinated COVID-19 patients in Cadiz, Spain, during the first and second waves of the pandemic.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2076-2607
YR 2023
FD 2023
LK http://hdl.handle.net/10498/32425
UL http://hdl.handle.net/10498/32425
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026