RT conference output T1 Taxonomic singularities of the veil of Flor yeasts in Fino Wines from the D.O. Jerez-Xérèz-Sherry A1 Cantoral Fernández, Jesús Manuel A1 Ruiz Muñoz, Marina A1 Hernández Fernández, María A1 Florido-Barba, Antonio A1 Villanueva Llanes, María de la Paz A1 Pizzi, Simona A1 Cordero Bueso, Gustavo Adolfo A2 BiomedicinaBiotecnología y Salud Pública AB The veil of “Flor yeasts” are responsible for the biological aging process of wines produced in theD.O. of Jerez and Sanlúcar de Barrameda (Finos and Manzanillas) in the province of Cádiz and Fino in theD.O. Montilla-Moriles in the province of Córdoba, as well as in other wine-growing regions of the world, suchas France, South Africa, California, Sardinia or Hungary. The first attempts to classify these flor veil yeastswere based on biochemical assays of sugar assimilation and fermentation. Thus, it was concluded that theyeasts involved in biological aging belonged all to Saccharomyces cerevisiae, distinguishing four subspecies orphysiological races: beticus, cheresiensis, montuliensis and rouxii. On the other hand, molecular techniques,which allow the characterization of oenological yeasts and discriminate between the different strains, areusually pulsed field electrophoresis (PFGE), the analysis of the polymorphism for the length of the restrictionfragments (RFLP) of mitochondrial DNA, and multiplex PCR of microsatellites. Recent studies in ourlaboratory show the existence of at least 9 different genotypes of S. cerevisiae veil of flor species, based on thestudy of microsatellites (SSR-PCR). In addition, we have detected the presence of some species of nonSaccharomyces yeasts not described previously. PB EDP Sciences SN 2117-4458 YR 2023 FD 2023 LK http://hdl.handle.net/10498/32638 UL http://hdl.handle.net/10498/32638 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 05-nov-2026