RT journal article
T1 Magnetic coagulometry: towards a new nanotechnological tool for ex vivo monitoring coagulation in human whole blood
A1 Santana Otero, Antonio
A1 Harper, A.
A1 Telling, L.
A1 Ortega Ponce, Daniel
A1 Cabrera, David
A2 Física de la Materia Condensada
K1 ROTATIONAL THROMBOELASTOMETRY ROTEM
K1 INTRAVENOUS HEPARIN
K1 NANOPARTICLES
K1 RIVAROXABAN
K1 BIVALIRUDIN
K1 HEMORRHAGE
K1 THERAPY
K1 SAFETY
K1 COST
K1 TIME
AB Blood clotting disorders consisting of unwanted blood clot formation or excessive bleeding are some of the main causes of death worldwide. However, there are significant limitations in the current methods used to clinically monitor the dynamics of clot formation in human whole blood ex vivo. Here a new magnetic coagulometry platform for testing ex vivo coagulation is described. This platform exploits the sensitivity of the out-of-phase component of alternating current (AC) magnetic susceptibility (χ′′) to variations in mobility and agglomeration of magnetic nanoparticles when trapped during blood clot formation. By labelling human whole blood with magnetic nanoparticles, the out-of-phase component of AC magnetic susceptibility shows that the dynamics of blood clot formation correlates with a decrease in the out-of-phase component χ′′ over time activation of coagulation. This is caused by a rapid immobilisation of nanoparticles upon blood coagulation and compaction. In contrast, this rapid fall in the out-of-phase component χ′′ is significantly slowed down when blood is pre-treated with three different anticoagulant drugs. Remarkably, the system showed sensitivity towards the effect of clinically used direct oral anticoagulation (DOAC) drugs in whole blood coagulation, in contrast to the inability of clinical routine tests prothrombin time (PT) and partial thromboplastin time (PTT) to efficiently monitor this effect. Translation of this nanomagnetic approach into clinic can provide a superior method for monitoring blood coagulation and improve the efficiency of the current diagnostic techniques.
PB ROYAL SOC CHEMISTRY
SN 2040-3372
YR 2023
FD 2023
LK http://hdl.handle.net/10498/32650
UL http://hdl.handle.net/10498/32650
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026