RT journal article T1 Optimisation of anaerobic co-digestion in two-stage systems for hydrogen, methane and biofertiliser production A1 Sillero Moreno, Leonor María A1 Pérez García, Montserrat A1 Solera del Río, María del Rosario K1 Biohydrogen K1 Biomethane K1 Biofertilizer K1 Anaerobic co-digestion K1 Sewage sludge K1 Vinasse and poultry manure AB This study responds to the need to optimise anaerobic digestion to achieve the integral valorisation of waste in asustainable way, obtaining maximum yields in the production of biofuels and biofertilisers. To achieve themaximum benefits of this technology, anaerobic co-digestion with phase separation by temperature and microorganisms (TPAD) of sewage sludge and waste from the agri-food sector (poultry manure and vinasse) wastested. To this end, each phase was optimised separately by applying different hydraulic retention times (HRT)first in the thermophilic acidogenic phase and then in the mesophilic methanogenic phase, in order to find theoptimum conditions in terms of hydrogen and methane yield, volatile solids (VS) removal and pathogen removalin the effluent. The results obtained show a maximum hydrogen yield of 40.41mLH2/gVS in the acidogenic phaseat 5 days of HRT and a methane yield of 391.0mLCH4/gVS in the mesophilic methanogenic phase at 12 days ofHRT, respectively. At the end of the process, a total removal of VS of 93 % and of pathogens of more than 96.7 %was achieved, which would allow it to be classified as class A biosolid according to USEPA. Therefore, thistechnology would allow solving a local environmental problem of organic waste generation, valorising it in theform of biohydrogen, biomethane and biofertiliser, thus contributing to the transfer to a circular economy model. SN 0016-2361 YR 2024 FD 2024-02-16 LK http://hdl.handle.net/10498/32689 UL http://hdl.handle.net/10498/32689 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 08-nov-2026