RT journal article
T1 Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration
A1 Munguba, Hermany
A1 Nikouei, H.
A1 Hochgerner, H.
A1 Oberst, P.
A1 Kouznetsova, A.
A1 Ryge, J.
A1 Muñoz Manchado, Ana Belén
A1 Close, J.
A1 Batista-Brito, Renata
A1 Linnarsson, S.
A1 Hjerling-Leffler, J.
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
K1 CELL-TYPES
K1 GABAERGIC INTERNEURONS
K1 SUPER-ENHANCERS
K1 SKELETAL-MUSCLE
K1 GENE-EXPRESSION
K1 RNA-SEQ
K1 SOX6
K1 DIVERSITY
K1 FATE
K1 LAYER
AB Although cardinal cortical interneuron identity is established upon cell-cycle exit, it remains unclear whether specific interneuron subtypes are pre-established, and if so, how their identity is maintained prior to circuit integration. We conditionally removed Sox6 (Sox6-cKO) in migrating somatostatin (Sst+) interneurons and assessed the effects on their mature identity. In adolescent mice, five of eight molecular Sst+ subtypes were nearly absent in the Sox6-cKO cortex without a reduction in cell number. Sox6-cKO cells displayed electrophysiological maturity and expressed genes enriched within the broad class of Sst+ interneurons. Furthermore, we could infer subtype identity prior to cortical integration (embryonic day 18.5), suggesting that the loss in subtype was due to disrupted subtype maintenance. Conversely, Sox6 removal at postnatal day 7 did not disrupt marker expression in the mature cortex. Therefore, Sox6 is necessary during migration for maintenance of Sst+ subtype identity, indicating that subtype maintenance requires active transcriptional programs.
PB CELL PRESS
SN 1097-4199
YR 2023
FD 2023
LK http://hdl.handle.net/10498/32727
UL http://hdl.handle.net/10498/32727
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026