RT journal article
T1 Induction of Oxidative Stress by Waterborne Copper and Arsenic in Larvae of European Seabass (Dicentrarchus labrax L.): A Comparison with Their Effects as Nanoparticles
A1 Torronteras, Rafael
A1 Díaz de Alba, Margarita Isabel
A1 Granado Castro, María Dolores
A1 Espada Bellido, Estrella
A1 Córdoba García, Francisco
A1 Canalejo, Antonio
A1 Galindo Riaño, María Dolores
A2 Química Analítica
K1 arsenic nanoparticles
K1 copper nanoparticles
K1 environmental pollutants;
K1 fish
K1 trace elements
AB The aim of this work was to compare the potential induction of oxidative stress and theantioxidant enzymatic response after a short-term waterborne exposure to copper (Cu) and arsenic(As) with that of the nanoparticles (NPs) of these elements (Cu-NPs and As-NPs) in fish larvae ofthe species Dicentrarchus labrax. Larvae were grouped in several tanks and exposed to differentconcentrations of contaminants (0 to 10 mg/L) for 24 or 96 h under laboratory conditions. Copperand arsenic concentrations were analysed in larval tissues using ICP-MS. A set of oxidative stressbiomarkers, including the levels of hydroperoxides (HPs), and superoxide dismutase (SOD) andcatalase (CAT) activities were assessed. The trace element concentrations (mg/kg d.w.) in larvaeranged as follows: 3.28–6.67 (Cu at 24 h) and 2.76–3.42 (Cu at 96 h); 3.03–8.31 (Cu-NPs at 24 h) and2.50–4.86 (Cu-NPs at 96 h); 1.92–3.45 (As at 24 h) and 2.22–4.71 (As at 96 h); and 2.19–8.56 (As-NPsat 24 h) and 1.75–9.90 (As-NPs at 96 h). In Cu tests, the oxidative damage (ROOH levels) wasinduced from 0.1 mg/L at both exposure times, while for Cu-NPs, this damage was not observeduntil 1 mg/L, which was paralleled by concomitant increases in SOD activity. The CAT activity wasalso increased but at lower metal concentrations (0.01 mg/L and 0.1 mg/L for both chemical forms).No oxidative damage was observed for As or As-NPs after 24 h, but it was observed for As after96 h of treatment with 0.01 mg/L. A decrease in SOD activity was observed for As after 24 h, but itturned out to be increased after 96 h. However, As-NPs did not alter SOD activity. The CAT activitywas stimulated only at 96 h by As and at 24 h by As-NPs. Therefore, the two chemical forms of Cuexhibited a higher bioaccumulation and toxicity potential as compared to those of As. Importantly, theassociation of both Cu and As in NPs reduced the respective trace metal bioaccumulation, resultingalso in a reduction in the toxic effects (mortality and biochemical). Furthermore, the assessment ofoxidative stress-related biomarkers in seabass larvae appears to be a useful tool for biomonitoringenvironmental-occurring trace elements.
PB MDPI
SN 2305-6304
YR 2024
FD 2024-02-09
LK http://hdl.handle.net/10498/32739
UL http://hdl.handle.net/10498/32739
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026