RT journal article
T1 Innovative Multipolymer-Based Electrochemical Biosensor Built on a Sonogel–Carbon Electrode Aiming for Continuous and Real-Time Lactate Determination in Physiological Samples: A New Scenario to Exploit Additive Printing †
A1 Sierra Padilla, Alfonso
A1 García Guzmán, Juan José
A1 Blanco Díaz, Lorena
A1 Bellido Milla, Dolores
A1 Palacios Santander, José María
A1 Cubillana Aguilera, Laura
A2 Química Analítica
K1 lactate biosensor
K1 sonogel–carbon
K1 gold sono-nanoparticles
K1 microfluidic cell
K1 3D printing
AB In this work, an amperometric biosensor for lactate determination based on a sonogel–carbon transducer has been developed and evaluated using the lactate oxidase enzyme coated with a multipolymer layer as a bioreceptor. The biosensor obtained had adequate sensitivity (4.16 × 10−8 A mM−1) and a wide linear working range (0.2–20 mM) that allowed for the determination of lactate at high concentrations without showing enzyme saturation phenomena. The selectivity of the biosensor was also verified using interferents commonly observed in physiological samples. Moreover, a microfluidic cell was designed and fabricated to allow the determination of lactate with the proposed biosensor in a continuous regime. In the end, the viability of the biosensor was tested with the proposed flow system using synthetic samples, obtaining excellent results.
PB MDPI
SN 2673-4591
YR 2023
FD 2023-09-26
LK http://hdl.handle.net/10498/32741
UL http://hdl.handle.net/10498/32741
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026