RT journal article
T1 Bioconversion of the invasive seaweed Rugulopteryx okamurae into enzymes and polyhydroxyalkanoates
T2 Bioconversion of the invasive seaweed Rugulopteryx okamurae into enzymes and polyhydroxyalkanoates
A1 Romero Vargas, Agustín
A1 Cala, Kelly
A1 Blandino Garrido, Ana María
A1 Díaz Sánchez, Ana Belén
A2 Ingeniería Química y Tecnología de Alimentos
K1 Invasive macroalgae
K1 Biological pretreatment
K1 SSF
K1 Hydrolytic enzymes
K1 PHA
AB The invasive macroalgae Rugulopteryx okamurae has been used as raw material for the production of hydrolytic enzymes and polyhydroxyalkanoates (PHA), bioplastic precursors. The fungus Aspergillus oryzae, grown in solid state fermentation (SSF) on the macroalga biomass, produced several hydrolytic enzymes, mainly xylanase and alginate lyase. Also, SSF was used as biological pretreatment of the macroalga to enhance the release of fermentable sugars after enzyme hydrolysis for subsequent PHA production. To increase sugar concentration and reduce the total cost of the process, the enzyme hydrolysis was performed at a high solid load and stirring rate, reducing the enzyme cocktail dose used by 63.5 %. At these conditions, the hydrolysate contained 18.52 g/L of reducing sugars, which were fermented with Cupriavidus necator, reaching a maximum of 288.0 mg PHA/L at 24 h.
SN 2211-9264
YR 2024
FD 2024-06-22
LK http://hdl.handle.net/10498/32754
UL http://hdl.handle.net/10498/32754
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026