RT journal article
T1 Polyhydroxybutyrate Production from the Macroalga Rugulopteryx okamurae: Effect of Hydrothermal Acid Pretreatment
A1 Romero Vargas, Agustín
A1 Blandino Garrido, Ana María
A1 Díaz Sánchez, Ana Belén
A1 Fernández Güelfo, Luis Alberto
A2 Ingeniería Química y Tecnología de Alimentos
A2 Tecnologías del Medio Ambiente
K1 macroalgae
K1 pretreatment
K1 enzyme hydrolysis
K1 fermentation
K1 Polyhydroxybutyrate
AB This study focuses on mitigating the socio-economic and environmental damage of the invasive macroalga Rugulopteryx okamurae and counteracting the pollution from petroleum-based plastics by using the alga as a feedstock for polyhydroxybutyrate (PHB) production. The enzymatic hydrolysis of R. okamurae, non-pretreated and hydrothermally acid-pretreated (0.2 N HCl, 15 min), was carried out, reaching reducing sugar (RS) concentrations of 10.7 g/L and 21.7 g/L, respectively. The hydrolysates obtained were used as a culture medium for PHB production with Cupriavidus necator, a Gram-negative soil bacterium, without supplementation with any external carbon and nitrogen sources. The highest yield (0.774 g PHB/g RS) and biopolymer accumulation percentage (89.8% cell dry weight, CDW) were achieved with hydrolysates from pretreated macroalga, reaching values comparable to the highest reported in the literature. Hence, it can be concluded that hydrolysates obtained from algal biomass hydrothermally pretreated with acid have a concentration of sugars and a C/N ratio that favour PHB production.
PB MDPI
YR 2024
FD 2024-07-21
LK http://hdl.handle.net/10498/32986
UL http://hdl.handle.net/10498/32986
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026