RT journal article
T1 Proteomic profiling of Botrytis cinerea conidial germination
A1 González Rodríguez, Victoria Eugenia
A1 Liñeiro, Eva
A1 Colby, Thomas
A1 Harzen, Anne
A1 Garrido Crespo, Carlos
A1 Cantoral Fernández, Jesús Manuel
A1 Schmidt, Jurgen
A1 Fernández Acero, Francisco Javier
A2 BiomedicinaBiotecnología y Salud Pública
K1 Botrytis cinerea
K1 Virulence factors
K1 Pathogenicity factors
K1 Fungal phytopathogens
K1 Fungal proteomics
K1 Conidia germination
AB Botrytis cinerea is one of the most relevantplant pathogenic fungi. The first step during its infectionprocess is the germination of the conidia. Here, we reporton the first proteome analysis during the germination of B.cinerea conidia, where 204 spots showed significant differencesin their accumulation between ungerminated andgerminated conidia by two-dimensional polyacrylamidegel electrophoresis and qPCR. The identified proteins weregrouped by gene ontology revealing that the infective toolsare mainly preformed inside the ungerminated conidiaallowing a quick fungal development at the early stages ofconidial germination. From 118 identified spots, severalvirulence factors have been identified while proteins, suchas mannitol-1-phosphate dehydrogenase, 6,7-dimethyl-8-ribityllumazine synthase or uracil phosphoribosyltransferase,have been disclosed as a new potential virulence factors in botrytis whose role in pathogenicity needs to be studied to gain new insights about the role of these proteinsas therapeutic targets and virulence factors.
PB Springer Link
SN 1432-072X
YR 2014
FD 2014-08-21
LK http://hdl.handle.net/10498/33261
UL http://hdl.handle.net/10498/33261
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026