RT journal article
T1 The F-actin capping protein is required for hyphal growth and full virulence but is dispensable for septum formation in Botrytis cinerea
A1 González Rodríguez, Victoria Eugenia
A1 Garrido Crespo, Carlos
A1 Cantoral Fernández, Jesús Manuel
A1 Schumacher, Julia
A2 BiomedicinaBiotecnología y Salud Pública
K1 Actin
K1 cytoskeleton
K1 Cell polarity
K1 Conidial germination
K1 Infection Morphogenesis
AB Filamentous (F-) actin is an integral part of the cytoskeleton allowing for cell growth, intracellularmotility, and cytokinesis of eukaryotic cells. Its assembly from G-actin monomersand its disassembly are tightly regulated processes involving a number of actin-bindingproteins (ABPs) such as F-actin nucleators and cross-linking proteins. F-actin capping protein(CP) is an alpha/beta heterodimer known from yeast and higher eukaryotes to bind tothe fast growing ends of the actin filaments stabilizing them. In this study, we identifiedthe orthologs of the two CP subunits, named BcCPA1 and BcCPB1, in the plant pathogenicfungus Botrytis cinerea and showed that the two proteins physically interact in a yeast twohybridapproach. GFP-BcCPA1 fusion proteins were functional and localized to the assumedsites of F-actin accumulation, i.e. to the hyphal tips and the sites of actin ring formation.Deletion of bccpa1 had a profound effect on hyphal growth, morphogenesis, andvirulence indicating the importance of F-actin capping for an intact actin cytoskeleton.As polarized growth e unlike septumformation e is impaired in the mutants, it can be concludedthat the organization and/or localization of actin patches and cables are disturbedrather than the functionality of the actin rings.
PB Elsevier
SN 1878-6146
YR 2016
FD 2016-07-22
LK http://hdl.handle.net/10498/33286
UL http://hdl.handle.net/10498/33286
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 15-nov-2026