RT journal article
T1 Simplification of iron speciation in wine samples: A spectrophotometric approach.
A1 López López, José Antonio
A1 Albendín García, María Gemma
A1 Arufe Martínez, María Isabel
A1 Manuel Vez, Pedro Manuel
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
A2 Química Analítica
K1 Iron
K1 inorganic iron
K1 speciation
K1 spectrophotometric
K1 wine
K1 DPKPH
AB A simple direct spectrophotometric method has been developed for analysis of Fe(II) and total Fe, in wine samples. This method is based on the formation of an Fe(II) complex with 2,2´-dipyridylketone picolinoylhydrazone (DPKPH), which shows a maximum green-blue absorption (l=700 nm) at pH 4.9.  Operative conditions for the batch procedure were investigated including reagent concentration, buffer solutions and wavelength used for the spectrophotometric detection. The tolerance limits of foreign ions and sample matrix have been also evaluated. Limits of detection and quantification were 0.005 mg L-1 and 0.017 mg L-1 of Fe(II) respectively, allowing its determination in real wine samples. Finally, the proposed method was used in the analysis of a series of white, rose and red wines. Results were compared with those obtained by the reference method Commission Regulation (ECC) No 2676/90 of September 1990 determining Community methods for the analysis of wines for Fe analysis, showing the reliability of the spectrophotometric determination of Fe with DPKPH in wine samples.
PB ACS Publications (USA)
SN ISSN: 0021-8561
YR 2015
FD 2015
LK http://hdl.handle.net/10498/33293
UL http://hdl.handle.net/10498/33293
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026