RT journal article
T1 Characterization and in vitro sensitivity of cholinesterases of gilthead seabream (Sparus aurata) to organophospate pesticides.
A1 Albendín García, María Gemma
A1 Arellano López, Juana María
A1 Manuel Vez, Pedro Manuel
A1 Sarasquete Reiriz, María del Carmen
A1 Arufe Martínez, María Isabel
A2 Anatomía PatológicaBiología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología
K1 Gilthead seabream
K1 Sparus aurata
K1 Biomarker
K1 Organophosphate
K1 Cholinesterase
K1 Inhibition kinetics
AB The characterization of cholinesterase activity in brain and muscle of gilthead seabream was carried out using four specific substrates and three selective inhibitors. In addition, Km and Vmax were calculated from the Michaelis-Menten equation for ASCh and BSCh substrates. Finally, the in vitro sensitivity of brain and muscle cholinesterases to three organophosphates was also investigated by estimating inhibition kinetics. The results indicate that, AChE is the enzyme present in the brain, whereas in muscle, a typical AChE form is present along with an atypical form of BChE. Very low ChE activity was found in plasma with all substrates used. The Inhibitory potency of the studied OPs on brain and muscle AChEs, based on bimolecular inhibition constants (ki) was: omethoate < dichlorvos < azinphosmethyl-oxon. Furthermore, muscle BChE was found to be several orders of magnitude (from 2 to 4) more sensitive than brain and muscle AChE inhibition by dichlorvos and omethoate
PB Springer (Holanda)
SN 0920-1742
YR 2017
FD 2017
LK http://hdl.handle.net/10498/33296
UL http://hdl.handle.net/10498/33296
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026