RT journal article T1 Olive tree pruning as an agricultural residue for ethanol production. Fermentation of hydrolysates from dilute acid pretreatment. A1 Díaz Villanueva, Manuel Jesús A1 Cara-Corpas, Cristobal A1 Ruiz-Ramos, Encarnación A1 Romero-Pulido, Inmaculada A1 Castro, Eulogio A2 Ingeniería Química y Tecnología de Alimentos K1 bioethanol K1 olive tree biomass K1 pentoses K1 Pichia stipitis K1 Pachysolen tannophilus AB The use of agricultural residues for ethanol production constitutes one of the most promising alternatives from an environmental point of view for substituting fossil fuels in the transport sector. This work deals with the fermentability of hydrolysates obtained from olive tree pruning biomass and the influence of the pH of the culture medium. Hydrolysates of olive tree biomass were obtained by dilute acid pre-treatment of the raw material at 180 °C and 1% (w/v) sulfuric acid concentration. After pretreatment, solid residue and liquid were separated by filtration. The liquid fraction (hydrolysate) was then submitted to detoxification (overliming) before being used as fermentation medium. Pichia stipitis and Pachysolen tannophilus were compared as fermenting microorganisms. Different initial pH values were also tested. The best results in terms of ethanol yield were obtained by P. tannophilus with values as high as 0.44 g ethanol g–1 sugar, and all liquids were fermented, to a different extent. P. stipitis could not ferment hydrolysates with initial pH below 6.5. It was also determined that ethanol production did not improve once glucose in the medium was totally converted, even if other sugars (xylose) were also consumed. PB Editorial CSIC SN 2171-9292 YR 2012 FD 2012 LK http://hdl.handle.net/10498/33300 UL http://hdl.handle.net/10498/33300 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 04-nov-2026