RT journal article T1 Organosolv pretreatment of olive tree biomass for fermentable sugar production A1 Díaz Villanueva, Manuel Jesús A1 Huijgen, W.J.J. A1 van der Laan, R.R. A1 Cara-Corpas, Cristobal A1 Castro, Eulogio K1 enzymatic hydrolysis K1 fermentable sugars K1 olive tree pruning biomass K1 organosolv pretreatment AB Olive tree pruning biomass is one of the main agricultural residues available in Mediterranean countries and is currently lacking commercial applications. For taking advantage of its sugar content, a pretreatment is necessary to enhance enzyme accessibility of the residue‟s cellulose fraction. This work describes for the first time the use of organosolv pretreatment for this purpose. The main process variables such as pretreatment temperature, residence time and solvent composition (aqueous ethanol) are studied. Results show that organosolv pretreatment causes delignification and hydrolysis of hemicelluloses and improves the enzymatic digestibility of olive tree pruning biomass. A higher pretreatment severity and ethanol content of the solvent were found to increase delignification (up to 64% at 66% w/w aqueous ethanol, 210°C, 60 min). On the other hand, xylan hydrolysis was promoted by a lower ethanol content (maximum 92%). The highest enzymatic hydrolysis yield (90% of the structural glucan present in the raw material) has been obtained after pretreatment with 43% w/w aqueousethanol at 210°C for 15 min. Organosolv was found to be the most effective pretreatment method for enzymatic hydrolysis of olive tree pruning biomass studied thus far. PB De Gruyter SN 0018-3830 YR 2011 FD 2011 LK http://hdl.handle.net/10498/33302 UL http://hdl.handle.net/10498/33302 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 08-nov-2026