RT journal article
T1 Inhibition of Pichia stipitis fermentation of hydrolysates from olive tree cuttings
A1 Díaz Villanueva, Manuel Jesús
A1 Ruiz-Ramos, Encarnación
A1 Romero-Pulido, Inmaculada
A1 Cara-Corpas, Cristobal
A1 Moya, Manuel
A1 Castro, Eulogio
A2 Ingeniería Química y Tecnología de Alimentos
K1 detoxification
K1 ethanol
K1 fermentation inhibition
K1 olive tree biomass
K1 Pichia stipitis
AB The ethanolic fermentation of liquid fractions (hydrolysates) issued from dilute acid pre-treatment ofolive tree biomass by Pichia stipitis is reported for the first time. On the one side, P. stipitis has beenreported as the most promising naturally occurring C5 fermenting microorganism; on the other side,olive tree biomass is a renewable, low cost, and lacking of alternatives agricultural residue especiallyabundant in Mediterranean countries. The study was performed in two steps. First, the fermentationperformance of P. stipitis was evaluated on a fermentation medium also containing the main inhibitorsfound in these hydrolysates (acetic acid, formic acid, and furfural), as well as glucose and xylose ascarbon sources. The effect of inhibitors, individually or in a mixture, on kinetic and yield parameterswas calculated. In a second step, hydrolysates obtained from 1% (w/w) sulfuric acid pre-treatment ofolive tree biomass at 190ºC for 10 min were used as a real fermentation medium with the samemicroorganism. Due to inhibition, effective fermentation required dilution of the hydrolysate andeither overliming or activated charcoal treatment. Results show that ethanol yields obtained fromhydrolysates, ranging from 0.35 to 0.42 g/g, are similar to those from synthetic medium, although theprocess proceeds at lower rates. Inhibiting compounds affect the fermentation performance in asynergistic way. Furfural is rapidly assimilated by the yeast; acetic acid and formic acidconcentrations decrease slowly during the process. Activated charcoal or overliming detoxificationimprove the fermentability of diluted hydrolysates.
PB Springer
SN 0959-3993
YR 2009
FD 2009
LK http://hdl.handle.net/10498/33306
UL http://hdl.handle.net/10498/33306
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 04-nov-2026