RT journal article
T1 Olfactory Neuroepithelium Cells from Cannabis Users Display Alterations to the Cytoskeleton and to Markers of Adhesion, Proliferation and Apoptosis
A1 Delgado Sequera, Alejandra Cristina
A1 Hidalgo Figueroa, María
A1 Barrera-Conde, Marta
A1 Durán Ruiz, María del Carmen
A1 Castro González, Carmen
A1 Fernández-Avilés, Cristina
A1 de la Torre, Rafael
A1 Sánchez Gomar, Ismael
A1 Pérez, Víctor
A1 Geribaldi Doldán, Noelia
A1 Robledo, Patricia
A1 Berrocoso Domínguez, Esther María
A2 Anatomía y Embriología Humana
A2 BiomedicinaBiotecnología y Salud Pública
A2 Psicología
K1 Cannabis
K1 Human olfactory Neuroepithelium
K1 Proteomic
K1 Cytoskeleton
K1 Adhesion
K1 Apoptosis
AB Cannabis is the third most commonly used psychoactive substance of abuse, yet it also receives considerable attentionas a potential therapeutic drug. Therefore, it is essential to fully understand the actions of cannabis in the human brain.The olfactory neuroepithelium (ON) is a peripheral nervous tissue that represents an interesting surrogate model tostudy the effects of drugs in the brain, since it is closely related to the central nervous system, and sensory olfactoryneurons are continually regenerated from populations of stem/progenitor cells that undergo neurogenesis throughoutlife. In this study, we used ON cells from chronic cannabis users and healthy control subjects to assess alterations inrelevant cellular processes, and to identify changes in functional proteomic pathways due to cannabis consumption.The ON cells from cannabis users exhibited alterations in the expression of proteins that were related to the cytoskeleton,cell proliferation and cell death, as well as, changes in proteins implicated in cancer, gastrointestinal andneurodevelopmental pathologies. Subsequent studies showed cannabis provoked an increase in cell size and morphologicalalterations evident through β-Tubulin III staining, as well as, enhanced beta-actin expression and a decrease inthe ability of ON cells to undergo cell attachment, suggesting abnormalities of the cytoskeleton and cell adhesionsystem. Furthermore, these cells proliferated more and underwent less cell death. Our results indicate that cannabismay alter key processes of the developing brain, some of which are similar to those reported in mental disorders likeDiGeorge syndrome, schizophrenia and bipolar disorder.
PB Springer
SN 1559-1182
YR 2021
FD 2021
LK http://hdl.handle.net/10498/33363
UL http://hdl.handle.net/10498/33363
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026