RT journal article T1 Impact of Sequential Inoculation with the Non- Saccharomyces T. delbrueckii and M. pulcherrima Combined with Saccharomyces cerevisiae Strains on Chemicals and Sensory Profile of Rosé Wines A1 Muñoz Redondo, José Manuel A1 Puertas, Belén A1 Cantos-Villar, Emma A1 Jiménez Hierro, María Jesús A1 Carbú Espinosa de los Monteros, María A1 Garrido Crespo, Carlos A1 Ruiz-Moreno, María José A1 Moreno-Rojas, José Manuel A2 BiomedicinaBiotecnología y Salud Pública K1 yeast K1 Sequential inoculation K1 róse wines K1 multivariate analysis K1 esters K1 sensory analysis K1 data fusion K1 non-Saccharomyces AB Controlled inoculations of non-Saccharomyces yeasts are becoming increasingly used to produce high-quality wines due to their enological potential. In this study, we evaluated the impact of sequential inoculation with the commercial non-Saccharomyces yeasts (Torulaspora delbrueckii and Metschnikowia pulcherrima) in combination with Saccharomyces cerevisiae on the chemical and sensory profile of rosé wines. Sequential inoculation with T. delbrueckii produced wines with an overall reduction in esters, mainly explained by the lower concentrations of ethyl esters of medium-chain fatty acids and isoamyl acetate. The lower ester concentrations of these wines were related to a reduction in fruity descriptors. An increase was observed, however, in other minor esters such as cinnamates and ethyl esters of branched acids. Zinc, ethyl isobutyrate, and ethyl dihydrocinnamate were selected as potential markers for this fermentation strategy. Sequential inoculation with M. pulcherrima resulted in rosé wines with an enhanced ester profile, reduced acetaldehyde, and increased anthocyans and tannins. Compared to the control wines fermented with S. cerevisiae, the changes observed in these wines were far subtler, especially for the volatile profile, sensory characteristics, and color parameters, with isobutyl hexanoate and isoamyl butyrate being selected as potential markers. SN 0021-8561 YR 2021 FD 2021 LK http://hdl.handle.net/10498/33401 UL http://hdl.handle.net/10498/33401 LA eng DS Repositorio Institucional de la Universidad de Cádiz RD 05-nov-2026