RT journal article
T1 Bioactivity and quantitative analysis of isohexenylnaphthazarins in root periderm of two Echium spp.: E. plantagineum and E. gaditanum
A1 García Durán, Alexandra
A1 Gutiérrez Vázquez, María Teresa
A1 Rial Cumbrera, Carlos
A1 Torres Martínez, Ascensión
A1 Varela Montoya, Rosa María
A1 Martínez Valdivia, Manuel Jesús
A1 González Molinillo, José María
A1 Skoneczny, Dominik
A1 A. Weston, Leslie
A1 Macías Domínguez, Francisco Antonio
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Orgánica
K1 Echium plantagineum
K1 Boraginaceae
K1 Naphthoquinones
K1 Bioactivity
K1 Quantification
K1 LC-MS-MS
AB Isohexenylnaphthazarins are commonly found in the root periderm of several Boraginaceous plants and are known for their broad range of biological activities. The work described herein concerns the biological activity of compounds from the roots of Echium plantagineum L. and Echium gaditanum Boiss (Boraginaceae) collected from field sites in southern Spain and Australia. Bioactivity was assessed using etiolated wheat coleoptile bioassay and in vitro growth inhibitory activity in HeLa and IGROV-1 cells. The quantification of four isohexenylnaphthazarins (shikonin/alkannin, deoxyshikonin/deoxyalkannin, acetylshikonin/acetylalkannin and dimethylacrylshikonin/dimethylacrylalkannin) was performed by LC-MS/MS using juglone as internal standard. Correlation coefficient values for the activities and concentrations of these four analytes were in the linear range and were greater than 0.99. Acetylshikonin/acetylalkannin and dimethylacrylshikonin/dimethylacrylalkannin were present in the highest concentrations in extracts of both species. The results reveal that greatest overall inhibition was observed in both bioassays with E. gaditanum extracts. Strong correlations between time of collection, sampling location and bioactivity were identified.
SN 0031-9422
YR 2017
FD 2017-09
LK http://hdl.handle.net/10498/33418
UL http://hdl.handle.net/10498/33418
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026