RT journal article
T1 Diversity of Interneurons in the Dorsal Striatum Revealed by Single-Cell RNA Sequencing and PatchSeq
A1 Muñoz Manchado, Ana Belen
A1 Bengtsson Gonzales, C.
A1 Zeisel, A.
A1 Munguba, H.
A1 Bekkouche, B.
A1 Skene, N.G.
A1 Lönnerberg, P.
A1 Ryge, J.
A1 Harris, K.D.
A1 Linnarsson, S.
A1 Hjerling-Leffler, Jens
K1 Pthlh
K1 cell classes
K1 cortex
K1 electrophysiology
K1 fast-spiking neurons
K1 interneuron
K1 parvalbumin
K1 single-cell RNA sequencing
K1 striatum
AB Striatal locally projecting neurons, or interneurons, act on nearby circuits and shape functional output to the rest of the basal ganglia. We performed sin- gle-cell RNA sequencing of striatal cells enriching for interneurons. We find seven discrete interneuron types, six of which are GABAergic. In addition to providing specific markers for the populations previ- ously described, including those expressing Sst/Npy, Th, Npy without Sst, and Chat, we identify two small populations of cells expressing Cck with or without Vip. Surprisingly, the Pvalb-expressing cells do not constitute a discrete cluster but rather are part of a larger group of cells expressing Pthlh with a spatial gradient of Pvalb expression. Using PatchSeq, we show that Pthlh cells exhibit a continuum of electro- physiological properties correlated with expression of Pvalb. Furthermore, we find significant molecular differences that correlate with differences in electro- physiological properties between Pvalb-expressing cells of the striatum and those of the cortex.
PB Cell Press
SN 2211-1247
YR 2018
FD 2018
LK http://hdl.handle.net/10498/33429
UL http://hdl.handle.net/10498/33429
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026