RT journal article
T1 Molecular Detection of Cryptosporidium spp. and Microsporidia in Human and Animal Stool Samples
A1 Gómez Romano, María Teresa
A1 Rodríguez Iglesias, Manuel Antonio
A1 Galán Sanchez, Fátima
A2 BiomedicinaBiotecnología y Salud Pública
K1 Cryptosporidiumspp
K1 Enterocytozoon bieneusi
K1 farm animals
K1 humans
K1 pets
K1 stool
AB Cryptosporidium spp. and Microsporidia are opportunistic microorganisms with remarkable zoonotic transmission potential due to their capacity to infect humans and animals. The aim of this study was to evaluate the prevalence of these microorganisms in stool samples of animal and human origin. In total, 369 stool samples (205 from human patients with diarrhea and 164 of animal origin) were included in the study. Cryptosporidium spp. and Microsporidia presence were determined by using multiplex nested PCR. Positive results were analyzed by using Sanger sequencing of the amplicon, utilizing BLASTN and ClustalX software to confirm identification. Cryptosporidium spp. were found in 0.97% and 4.26% of human and animal samples, respectively. Enterocytozoon bieneusi was detected in human and animal stools in 6.82% and 3.05% of the samples, respectively. No associations were found when analyzing the presence of Cryptosporidium spp. and E. bieneusi and the demographic and clinical variables of patients and animals. This study demonstrates the presence of these microorganisms in human and animal samples from different species, and the most interesting findings are the detection of Cryptosporidium spp. in pets (e.g., rodents) that are not usually included in this type of study, and the identification of E. bieneusi in patients with diarrhea without underlying disease.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2076-2607
YR 2024
FD 2024
LK http://hdl.handle.net/10498/33527
UL http://hdl.handle.net/10498/33527
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 14-nov-2026