RT journal article
T1 Proteomic analysis in the brain and liver of sea bream (Sparus aurata) exposed to the antibiotics ciprofloxacin, sulfadiazine, and trimethoprim
A1 Fernandez, R.
A1 Colás Ruiz, Nieves del Rocio
A1 Lara Martín, Pablo Antonio
A1 Fernández Cisnal, R.
A1 Hampel, Miriam
A1 Hampel
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Física
K1 Antibiotics
K1 Proteomics
K1 Sparus aurata
K1 Emerging pollutants
AB Antibiotics, frequently detected in aquatic ecosystems, can negatively impact the health of resident organisms.Although the study on the possible effects of antibiotics on these organisms has been increasing, there is still littleinformation available on the molecular effects on exposed non-target organisms. In our study we used a label freeproteomic approach and sea bream, Sparus aurata, to evaluate the effects of exposure to environmentally relevantconcentrations of the antibiotic compounds ciprofloxacin (CIP), sulfadiazine (SULF) and trimethoprim (TRIM)produced at the protein level. Individuals of sea bream were exposed to single compounds at 5.2 ± 2.1 μg L− 1 ofCIP, 3.8 ± 2.7 μg L− 1 of SULF and 25.7 ± 10.8 μg L− 1 of TRIM for 21 days. After exposure, the number ofdifferentially expressed proteins in the liver was 39, 73 and 4 for CIP, SULF and TRIM respectively. In the brain,there was no alteration of proteins after CIP and TRIM treatment, while 9 proteins were impacted after SULFtreatment. The differentially expressed proteins were involved in cellular biological, metabolic, developmental,growth and biological regulatory processes. Overall, our study evidences the vulnerability of Sparus aurata, afterexposure to environmentally relevant concentrations of the major antibiotics CIP, SULF and TRIM and that theirchronic exposure could lead to a stress situation, altering the proteomic profile of key organs such as brain andliver.
PB Elsevier Ltd
SN 1873-6424
YR 2024
FD 2024
LK http://hdl.handle.net/10498/33640
UL http://hdl.handle.net/10498/33640
LA eng
NO Ministerio de Economía y Competitividad (MINECO), SpainUniversidad de Cádiz, SpainAsociación Universitaria Iberoamericana de Postgrado, SpainMinisterio de Asuntos Económicos y Transformación Digital, Gobierno de España, SpainLatin American Association of Postgraduates
DS Repositorio Institucional de la Universidad de Cádiz
RD 22-sep-2026