RT journal article
T1 Optimization of the Enzymatic Extraction of Naringenin from Pink Grapefruit Pulp (Citrus × paradisi Macfad.)
A1 Polo Castellano, Curro
A1 Mateos Bernal, Rosa María
A1 Visiedo García, Francisco Manuel
A1 Palma Lovillo, Miguel
A1 Fernández Barbero, Gerardo
A1 Ferreiro González, Marta
A2 Anatomía y Embriología Humana
A2 BiomedicinaBiotecnología y Salud Pública
A2 Neurociencias
A2 Química Analítica
K1 Citrus × paradisi Lam
K1 enzymatic extraction
K1 Plackett–Burman
K1 Box–Behnken
K1 naringenin
AB Naringenin is one of the main phenolic compounds found in grapefruit (Citrus × paradisiMacfad.). This compound is known for its therapeutical properties as an antioxidant, antidiabetic,antihyperlipidemic and antineoplastic agent. In order to enable the development of drugs based onthis compound, an appropriate extraction method needs to be developed. For this study, enzymaticextraction was chosen, as it is a cheap and green extraction method. Optimal extraction conditions(pH, temperature, agitation, solvent composition, sample-to-solvent ratio and enzyme-to-sampleratio) were determined through a Plackett–Burman and a Box–Behnken design, resulting in pH 6.0,40 ◦C, 50 rpm, 20% EtOH, 0.2 g sample per 15 mL solvent and 1000 U/g. Once extraction conditionswere determined, a single-factor experiment was performed under optimal conditions to determineextraction time, which resulted in 10 min per extraction. Finally, repeatability and intermediateprecision were evaluated through naringenin quantification. Good values were obtained for bothparameters (1.80% and 2.05%, respectively). Furthermore, extracts presented significant amount ofnaringenin (0.18 ± 0.02 mg/g).
PB MDPI
SN 2073-4395
YR 2024
FD 2024
LK http://hdl.handle.net/10498/33707
UL http://hdl.handle.net/10498/33707
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 09-nov-2026