RT journal article
T1 Unravelling the Function of the Sesquiterpene Cyclase STC3 in the Lifecycle of Botrytis cinerea
A1 Coca Ruiz, Víctor
A1 Suárez Cáceres, Ivonne Rocío
A1 Aleu Casatejada, Josefina
A1 Cantoral Fernández, Jesús Manuel
A1 González, Celedonio
A1 Garrido Crespo, Carlos
A1 Brito, Nélida
A1 González Collado, Isidro
A2 BiomedicinaBiotecnología y Salud Pública
A2 Química Orgánica
K1 Botrytis cinerea
K1 sesquiterpene cyclase
K1 secondary metabolism
K1 metabolites and sesquiterpenes
AB The genome sequencing of Botrytis cinerea supplies a general overview of the map of genes involved in secondary metabolite synthesis. B. cinerea genomic data reveals that this phytopathogenic fungus has seven sesquiterpene cyclase (Bcstc) genes that encode proteins involved in the farnesyl diphosphate cyclization. Three sesquiterpene cyclases (BcStc1, BcStc5 and BcStc7) are characterized, related to the biosynthesis of botrydial, abscisic acid and (+)-4-epi-eremophilenol, respectively. However, the role of the other four sesquiterpene cyclases (BcStc2, BcStc3, BcStc4 and BcStc6) remains unknown. BcStc3 is a well-conserved protein with homologues in many fungal species, and here, we undertake its functional characterization in the lifecycle of the fungus. A null mutant ΔBcstc3 and an overexpressed–Bcstc3 transformant (OvBcstc3) are generated, and both strains show the deregulation of those other sesquiterpene cyclase-encoding genes (Bcstc1, Bcstc5 and Bcstc7). These results suggest a co-regulation of the expression of the sesquiterpene cyclase gene family in B. cinerea. The phenotypic characterization of both transformants reveals that BcStc3 is involved in oxidative stress tolerance, the production of reactive oxygen species and virulence. The metabolomic analysis allows the isolation of characteristic polyketides and eremophilenols from the secondary metabolism of B. cinerea, although no sesquiterpenes different from those already described are identified.
PB Multidisciplinary Digital Publishing Institute
SN 1661-6596
YR 2024
FD 2024
LK http://hdl.handle.net/10498/33737
UL http://hdl.handle.net/10498/33737
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026