RT journal article
T1 Anthocyanin extraction from roselle (Hibiscus sabdariffa L.) calyces: A microwave-assisted approach using Box-Behnken design
A1 Dewi Larasati, Intan
A1 Carrera Fernández, Ceferino Adrián
A1 Nuryani Lioe, Hanifah
A1 Estiasih, Teti
A1 Dewi Yuliana, Nancy
A1 Manikharda
A1 Ronald Daniel Ray, Hamidie
A1 Palma Lovillo, Miguel
A1 Setyaningsih, Widiastuti
A2 Química Analítica
K1 Box-behnken design
K1 Edible flower
K1 Green-extraction
K1 Microwave-assisted extraction
AB Roselle (Hibiscus sabdariffa L.) is a widely consumed edible flower, often utilized as a natural coloring agent. The bright red color of roselle is due to its anthocyanin content. Beyond their role as colorants, anthocyanins are known for their potent antioxidant properties, which have drawn significant interest for their potential health benefits. To fully harness the advantages of roselle’s anthocyanins, developing a reliable extraction method is crucial. This study aimed to establish a fast, efficient, and environmentally friendly extraction technique using microwave-assisted extraction (MAE). Key factors, such as temperature (30, 65, and 100 ◦C), solvent concentration (30 %, 50 %, and 70 % ethanol in water), and solvent-to-solid ratio (10:1, 25:1, 40:1), were optimized using the Box-Behnken design combined with response surface methodology. The extraction time (5–30 min) was also evaluated to maximize anthocyanin recovery. Two major anthocyanins, delphinidin-3-sambubioside and cyanidin-3-sambubioside, were identified using UHPLC-PDA-QToF-MS and quantified using a cyanidin-3-glucoside calibration curve. The optimal extraction conditions were found to be 72 ◦C, using 70 % ethanol in water with a 40:1 solvent-to-solid ratio for 15 min. This method was validated with high accuracy (>98 % recovery) and precision (CV <6 %). It was successfully applied to quantify the anthocyanin content in both purpleand red roselle varieties. This rapid and effective MAE method offers a more sustainable, energy-efficient, and time-saving alternative to conventional extraction methods, benefiting industries that rely on natural colorants and antioxidants.
SN 2666-1543
YR 2024
FD 2024-10-24
LK http://hdl.handle.net/10498/33782
UL http://hdl.handle.net/10498/33782
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026