RT journal article
T1 Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS
A1 Arca Suárez, Jorge
A1 Galán Sánchez, Fátima
A1 Marín Casanova, Pilar
A1 Rodríguez Iglesias, Manuel Antonio
A2 BiomedicinaBiotecnología y Salud Pública
AB We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratories.
PB Public Library of Science
SN 1932-6203
YR 2017
FD 2017
LK http://hdl.handle.net/10498/33966
UL http://hdl.handle.net/10498/33966
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026