RT journal article
T1 Development of a new eco-friendly ultrasound-assisted extraction method to quantify tryptophan in wild mushrooms and determination of its beneficial properties
A1 Ruiz López, Alejandro
A1 Ortega-Caneda, Elena
A1 Espada Bellido, Estrella
A1 Chinchilla Salcedo, Nuria
A1 Palma Lovillo, Miguel
A1 Aliaño González, María José
A1 Fernández Barbero, Gerardo
A1 Carrera Fernández, Ceferino Adrián
A2 Química Analítica
A2 Química Orgánica
K1 Bioactive compounds
K1 Tryptophan
K1 Mushrooms
K1 Ultrasound-assisted extraction
K1 Box–Behnken design
K1 Acetylcholinesterase
K1 Antioxidant capacity
AB Bioactive compounds in food offer health benefits by influencing cellular and physiological functions. Tryptophan,an essential amino acid and precursor to neurotransmitters like melatonin and serotonin, regulates moodand circadian rhythms. However, its quantification in mushrooms is scarce studied. An eco-friendly ultrasoundassisted extraction (UAE) method has been optimized to quantify tryptophan using ultra high-performance liquid chromatography with a diode array and fluorescence detector (UHPLC-DAD-Fl). Tryptophan levels were determined in 26 wild mushroom samples of the genus Lactarius and Boletus. The concentrations ranged from 0.042 mg/g to 0.742 mg/g. The extracts’ health benefits were assessed for antioxidant capacity using DPPH (17.7 % -71.6 %) and ABTS (7.2 % - 24.9 %) methods, while acetylcholinesterase (AChE) inhibitory activity ranged from13.1 % to 49.8 %. Promising results were obtained. Hierarchical cluster analysis demonstrated a correlationbetween tryptophan concentration, mushroom species, location, and extract properties, highlighting tryptophan’scrucial role in these mushrooms’ health benefits.
SN 0308-8146
YR 2024
FD 2024-11-12
LK http://hdl.handle.net/10498/34094
UL http://hdl.handle.net/10498/34094
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 07-nov-2026