RT journal article
T1 Synthetic mimetics of the endogenous gastrointestinal nanomineral: Silent constructs that trap macromolecules for intracellular delivery
A1 Pele, Laetitia C.
A1 Haas, Carolin T.
A1 Hewitt, Rachel E.
A1 Robertson, Jack
A1 Skepper, Jeremy
A1 Brown, Andy
A1 Hernández Garrido, Juan Carlos
A1 Midgley, Paul A.
A1 Faria, Nuno
A1 Chappell, Helen
A1 Powell, Jonathan J.
A2 Ciencia de los Materiales e Ingeniería Metalúrgica y Química Inorgánica
K1 Amorphous magnesium-substituted calcium phosphate
K1 Nanoparticles
K1 Peptidoglycan
K1 PD-L1
AB Amorphous magnesium-substituted calcium phosphate (AMCP) nanoparticles (75-150 nm) form constitutively in large numbers in the mammalian gut. Collective evidence indicates that they trap and deliver luminal macromolecules to mucosal antigen presenting cells (APCs) and facilitate gut immune homeostasis. Here, we report on a synthetic mimetic of the endogenous AMCP and show that it has marked capacity to trap macromolecules during formation. Macromolecular capture into AMCP involved incorporation as shown by STEM tomography of the synthetic AMCP particle with 5 nm ultra-fine iron (III) oxohydroxide. In vitro, organic cargo-loaded synthetic AMCP was taken up by APCs and tracked to lysosomal compartments. The AMCP itself did not regulate any gene, or modify any gene regulation by its cargo, based upon whole genome transcriptomic analyses. We conclude that synthetic AMCP can efficiently trap macromolecules and deliver them to APCs in a silent fashion, and may thus represent a new platform for antigen delivery.
PB Elsevier
SN 1549-9642
YR 2017
FD 2017
LK http://hdl.handle.net/10498/34165
UL http://hdl.handle.net/10498/34165
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 13-nov-2026