RT journal article
T1 Nitrate stimulation of indigenous nitrate-reducing, sulfide-oxidising bacterial community in wastewater anaerobic biofilms
A1 García de Lomas, Juan
A1 Corzo Rodríguez, Alfonso
A1 Portillo, Maria del Carmen
A1 Gonzalez, Juan
A1 Andrades, Jose A.
A1 Sainz-jimenez, Cesareo
A1 García Robledo, Emilio Guillermo
A2 Biología
K1 Wastewater
K1 Sulfide
K1 Biofilm
K1 Nitrate-reducing bacteria
AB The role of the nitrate-reducing, sulfide-oxidising bacteria (NR-SOB) in the nitrate-mediated inhibition of sulfide net production by anaerobic wastewater biofilms was analyzed in two experimental bioreactors, continuously fed with the primary effluent of a wastewater treatment plant, one used as control (BRC) and the other one supplemented with nitrate (BRN). This study integrated information from H2S and pH microelectrodes, RNA-based molecular techniques, and the time course of biofilm growth and bioreactors water phase. Biofilms were a net source of sulfide for the water phase (2.01 μmol S2−tot m−2 s−1) in the absence of nitrate dosing. Nitrate addition effectively led to the cessation of sulfide release from biofilms despite which a low rate of net sulfate reduction activity (0.26 μmol S2−tot m−2 s−1) persisted at a deep layer within the biofilm. Indigenous NR-SOB including Thiomicrospira denitrificans, Arcobacter sp., and Thiobacillus denitrificans were stimulated by nitrate addition resulting in the elimination of most sulfide from the biofilms. Active sulfate reducing bacteria (SRB) represented comparable fractions of total metabolically active bacteria in the libraries obtained from BRN and BRC. However, we detected changes in the taxonomic composition of the SRB community suggesting its adaptation to a higher level of NR-SOB activity in the presence of nitrate.
PB Elsevier
SN 0043-1354
YR 2007
FD 2007-04-09
LK http://hdl.handle.net/10498/34449
UL http://hdl.handle.net/10498/34449
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 14-nov-2026