RT journal article
T1 Gonadotropin-inhibitory hormone in the flatfish: molecular cloning, brain localization and physiological effects
A1 Aliaga Guerrero, María
A1 Paullada Salmerón, José Antonio
A1 Piquer, Vanesa
A1 Mañanos, E.L.
A1 Muñoz Cueto, José Antonio
A2 Biología
K1 brain
K1 flatfish
K1 Gnih
K1 immunohistochemistry
K1 LPXRF-amide
K1 mRNA expression
K1 neuropeptides
K1 pituitary
K1 reproduction
AB In our study, we focused on the gonadotropin-inhibitory hormone (GnIH) system in the Senegalese sole (Solea senegalensis), aiming to clarify its role in neuroendocrine and environmental control of reproduction. We successfully cloned the full-length Gnih precursor sequence, coding for three putative GnIH peptides (ssGnIH), and generated specific antibodies against these peptides. Using these antibodies, we localized GnIH cells and their projections in the brain and pituitary. We observed significant gnih expression in the diencephalon, as well as in the olfactory bulbs/cerebral hemispheres, optic tectum/tegmentum, retina, and pituitary. The antibodies consistently revealed ssGnIH-immunoreactive perikarya in the olfactory bulbs, ventral telencephalon, caudal preoptic area, dorsal tegmentum, and rostral rhombencephalon, with fibers innervating the brain and pituitary extensively. Functionally, intramuscular injection of ssGnIH-3 significantly reduced gnrh-3 and lh expression, while ssGnIH-2 had no effect on the main reproductive genes. These results indicate the presence of a functional GnIH system in the sole, with widespread innervation of brain areas and the pituitary gland. This system likely plays a crucial role in the neuroendocrine regulation of flatfish reproduction, providing new insights into the broader functions of GnIH in teleosts.
PB Wiley
YR 2018
FD 2018
LK http://hdl.handle.net/10498/34510
UL http://hdl.handle.net/10498/34510
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026