RT journal article
T1 On the influence of structural and chemical properties on the elastic modulus of woven bone under healing
A1 Blázquez Carmona, Pablo
A1 Mora Macías, Juan
A1 Pajares, Antonia
A1 Mármol, Álvaro
A1 Reina Romo, Esther
A2 Ingeniería Mecánica y Diseño Industrial
K1 woven bone
K1 nanoindentation
K1 micro-CT
K1 Raman sprectroscopy
K1 composition
K1 distraction osteogenesis
K1 elastic modulus
K1 power law
AB Introduction: Woven bone, a heterogeneous and temporary tissue in boneregeneration, is remodeled by osteoblastic and osteoclastic activity andshaped by mechanical stress to restore healthy tissue properties.Characterizing this tissue at different length scales is crucial for developingmicromechanical models that optimize mechanical parameters, therebycontrolling regeneration and preventing non-unions.Methods: This study examines the temporal evolution of the mechanicalproperties of bone distraction callus using nanoindentation, ash analysis,micro-CT for trabecular microarchitecture, and Raman spectroscopy formineral quality. It also establishes single- and two-parameter power lawsbased on experimental data to predict tissue-level and bulk mechanicalproperties.Results: At the macro-scale, the tissue exhibited a considerable increase in bonefraction, controlled by the widening of trabeculae. The Raman mineral-to-matrixratios increased to cortical levels during regeneration, but the local elasticmodulus remained lower. During healing, the tissue underwent changes in ashfraction and in the percentages of Calcium and Phosphorus. Six statisticallysignificant power laws were identified based on the ash fraction, bonefraction, and chemical and Raman parameters.Discussion: The microarchitecture of woven bone plays a more significant rolethan its chemical composition in determining the apparent elastic modulus of thetissue. Raman parameters were demonstrated to provide more significant powerlaws correlations with the micro-scale elastic modulus than mineral content fromash analysis.
PB Frontiers
SN 2296-4185
YR 2024
FD 2024-10-01
LK http://hdl.handle.net/10498/34550
UL http://hdl.handle.net/10498/34550
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026