RT journal article
T1 Cyclin C stimulates β-cell proliferation in rat and human pancreatic β-cells
A1 Jiménez Palomares, Margarita
A1 Lopez-Acosta, José Francisco
A1 Villa-Pérez, Pablo
A1 Moreno-Amador, José Luis
A1 Muñoz-Barrera, Jennifer
A1 Fernandez-Luis, Sara
A1 Heras-Pozas, Blanca
A1 Perdomo, Germán
A1 Bernal-Mizrachi, Ernesto
A1 Cozar-Castellano, Irene
A2 BiomedicinaBiotecnología y Salud Pública
K1 cell cycle
K1 cyclin C
K1 pancreatic β-cell
K1 proliferation
AB Activation of pancreatic β-cell proliferation has been proposed as an approach to replace reduced functional β-cell mass in diabetes. Quiescent fibroblasts exit from G0 (quiescence) to G1 through pRb phosphorylation mediated by cyclin C/cdk3 complexes. Overexpression of cyclin D1, D2, D3, or cyclin E induces pancreatic β-cell proliferation. We hypothesized that cyclin C overexpression would induce β-cell proliferation through G0 exit, thus being a potential therapeutic target to recover functional β-cell mass. We used isolated rat and human islets transduced with adenovirus expressing cyclin C. We measured multiple markers of proliferation: [(3)H]thymidine incorporation, BrdU incorporation and staining, and Ki67 staining. Furthermore, we detected β-cell death by TUNEL, β-cell differentiation by RT-PCR, and β-cell function by glucose-stimulated insulin secretion. Interestingly, we have found that cyclin C increases rat and human β-cell proliferation. This augmented proliferation did not induce β-cell death, dedifferentiation, or dysfunction in rat or human islets. Our results indicate that cyclin C is a potential target for inducing β-cell regeneration.
SN 1522-1555
YR 2015
FD 2015-03-15
LK http://hdl.handle.net/10498/34804
UL http://hdl.handle.net/10498/34804
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 10-nov-2026