RT journal article
T1 Wild grape-associated yeasts as promising biocontrol agents against Vitis vinifera fungal pathogens.
A1 Cordero Bueso, Gustavo Adolfo
A1 Mangieri, Nicola
A1 Maghradze, David
A1 Foschino, Roberto
A1 Valdetara, Federica
A1 Cantoral Fernández, Jesús Manuel
A1 Vigentini, Ileana
A2 BiomedicinaBiotecnología y Salud Pública
K1 yeasts
K1 molds
K1 vinifera ssp sylvestris
K1 biocontrol
K1 fungal diseases
AB The increasing level of hazardous residues in the environment and food chains hasled the European Union to restrict the use of chemical fungicides. Thus, exploitingnew natural antagonistic microorganisms against fungal diseases could serve theagricultural production to reduce pre- and post-harvest losses, to boost safer practicesfor workers and to protect the consumers’ health. The main aim of this work wasto evaluate the antagonistic potential of epiphytic yeasts against Botrytis cinerea,Aspergillus carbonarius, and Penicillium expansum pathogen species. In particular, yeastisolation was carried out from grape berries of Vitis vinifera ssp sylvestris populations,of the Eurasian area, and V. vinifera ssp vinifera cultivars from three different farmingsystems (organic, biodynamic, and conventional). Strains able to inhibit or slow thegrowth of pathogens were selected by in vitro and in vivo experiments. The mosteffective antagonist yeast strains were subsequently assayed for their capability tocolonize the grape berries. Finally, possible modes of action, such as nutrients andspace competition, iron depletion, cell wall degrading enzymes, diffusible and volatileantimicrobial compounds, and biofilm formation, were investigated as well. Two hundredand thirty-one yeast strains belonging to 26 different species were isolated; 20 ofthem, ascribed to eight species, showed antagonistic action against all molds. Yeastsisolated from V. vinifera ssp sylvestris were more effective (up to 50%) against B. cinerearather than those isolated from V. vinifera ssp vinifera. Six strains, all isolated from wildvines, belonging to four species (Meyerozyma guilliermondii, Hanseniaspora uvarum,Hanseniaspora clermontiae, and Pichia kluyveri) revealed one or more phenotypicalcharacteristics associated to the analyzed modes of antagonistic action
PB Frontiers
SN 1664-302X
YR 2017
FD 2017-11-03
LK http://hdl.handle.net/10498/34850
UL http://hdl.handle.net/10498/34850
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026