RT journal article
T1 Myrcia sylvatica essential oil mitigates molecular, biochemical and physiological alterations in Rhamdia quelen under different stress events associated to transport
A1 Saccol, Etiane M.H.
A1 Jerez Cepa, Ismael
A1 Ourique, Giovana M.
A1 Pês, Tanise S.
A1 Gressler, Luciane T.
A1 Mourão, Rosa H.V.
A1 Martínez-Rodríguez, Gonzalo
A1 Mancera Romero, Juan Miguel
A1 Baldisserotto, Bernardo
A1 Pavanato, Maria A.
A1 Martos Sitcha, Juan Antonio
A2 Biología
K1 corticotropin-releasing hormone
K1 gene expression
K1 natural products
K1 proopiomelanocortin
K1 sedation
AB The effects of pre-transport handling and addition of essential oil of Myrcia sylvatica (EOMS) during transport on stress pathways activation in Rhamdia quelen were investigated. Fish (n = 400, 25.2 ± 2.9 g) were captured in production ponds and transferred to 100-L tank (density 100 g L− 1). After 24 h, 10 fish were sampled (before transport group). The remaining fish were placed in plastic bags (n = 30 or 32 fish per bag, density 150 g L− 1) containing 5 L of water (control), ethanol (315 μL L− 1, vehicle) or EOMS (25 or 35 μL L− 1), in triplicate, transported for 6 h and sampled (n = 10 animals per group). Indicators of stress and metabolism, as well as mRNA expression of brain hormones were evaluated. Previously, full-length cDNAs, encoding specific corticotropin-releasing hormone (crh) and proopiomelanocortins (pomca and pomcb), were cloned from whole brain of R. quelen. Crh expression increased after 24 h of capture and handling, whereas cortisol and glucose plasmatics enhanced their values in the control group. Transport with EOMS reduced plasma cortisol and lactate levels, while ethanol and EOMS groups increased Na+/K+-ATPase gill activity compared to control. Gene expression of crh, pomcb, prolactin and somatolactin mRNAs were lower after transport with EOMS compared to control. EOMS was able to mitigate the stress pathways activation caused by transport, maintaining a balance in body homeostasis. Thus, EOMS is recommended as sedative in procedures as transport and the pre-transport handling requires greater attention and use of tranquilizers.
PB Elsevier
SN 1532-2661
YR 2018
FD 2018-04
LK http://hdl.handle.net/10498/34868
UL http://hdl.handle.net/10498/34868
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 08-nov-2026