RT journal article
T1 Rapid and not culture-dependent assay based on multiplex PCR-SSR analysis for monitoring inoculated yeast strains in industrial wine fermentations.
A1 Cordero Bueso, Gustavo Adolfo
A1 Rodríguez Jiménez, María Esther
A1 Garrido Crespo, Carlos
A1 Cantoral Fernández, Jesús Manuel
A2 BiomedicinaBiotecnología y Salud Pública
K1 Activated carbon
K1 DNA extraction
K1 Microsatellite
K1 Fermentation control
K1 Saccharomyces cerevisiae
AB Wine industry needs a simple method for rapid diagnosis of the dominance of inoculated strains that could be performed routinely during the fermentation process. We present a suitable, high-throughput, and low-cost method to monitor rapidly the dominance of inoculated yeast strains in industrial fermentations of red and white wines using an activated carbon cleaning pretreatment, and a rapid DNA extraction method plus multiplex PCR-SSR analysis. We apply this technique directly to samples of fermenting wines without previously isolating yeast colonies. Results are obtained in a maximum time of 4.5 h.
SN 1432-072X,
YR 2017
FD 2017-01-01
LK http://hdl.handle.net/10498/34929
UL http://hdl.handle.net/10498/34929
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 11-nov-2026