RT journal article
T1 Dual Enlargement of Gold Nanoparticles: From Mechanism to Scanometric Detection of Pathogenic Bacteria
A1 Cao, Cuong
A1 Cervera Gontard, Lionel
A1 Tram, Le Ly Thuy
A1 Wolff, Anders
A1 Bang, Dang Duong
A2 Física de la Materia Condensada
AB A mechanism of dual enlargement of gold nanoparticles (AuNPs) comprising twosteps is described. In the fi rst step, the AuNPs are enlarged by depositing Au atomson their crystalline faces. In this process, the particles are not only enlarged but theyare also observed to multiply: new Au nuclei are formed by the budding and divisionof the enlarged particles. In the second step, a silver enhancement is subsequentlyperformed by the deposition of silver atoms on the enlarged and newly formedAuNPs to generate bimetallic Au@Ag core–shell structures. The dual nanocatalysisgreatly enhances the electron density of the nanostructures, leading to a strongerintensity for colorimetric discrimination as well as better sensitivity for quantitativemeasurement. Based on this, a simple scanometric assay for the on-slide detectionof the food-born pathogen Campylobacter jejuni is developed. After capturing thetarget bacteria, gold-tagged immunoprobes are added to create a signal on a solidsubstrate. The signal is then amplifi ed by the dual enlargement process, resulting in astrong color intensity that can easily be recognized by the unaided eye, or measuredby an inexpensive fl atbed scanner. In this paper, dual nanocatalysis is reported for thefi rst time. It provides a valuable mechanistic insight into the development of a simpleand cost-effective detection format.
PB Wiley
SN 1613-6810
YR 2011
FD 2011
LK http://hdl.handle.net/10498/34997
UL http://hdl.handle.net/10498/34997
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026