RT journal article
T1 Obesity induced alterations in redox homeostasis and oxidative stress are present from an early age
A1 Lechuga Sancho, Alfonso María
A1 Gallego Andújar, David
A1 Ruiz Ocaña, Pablo
A1 Visiedo García, Francisco Manuel
A1 Sáez Benito, Ana
A1 Schwarz Rodríguez, Mónica
A1 Segundo Iglesias, Maria del Carmen
A1 Mateos Bernal, Rosa María
A2 Anatomía y Embriología Humana
A2 BiomedicinaBiotecnología y Salud Pública
K1 oxidative stress
K1 erythrocytes
K1 childhood obesity
AB ObjectivesOxidative stress and inflammation have been postulated as underlying mechanisms for the development of obesity-related insulin resistance. This association however, remains elusive especially in childhood. We sought to investigate this relation by measuring oxidative stress and antioxidant response biomarkers, before and during an oral glucose tolerancetest (OGTT), in different biological samples from obese children.Subjects24 children were recruited for the study, (18 obese and 6 controls). After OGTT, the obese group was subdivided in two, according to whether or not carbohydrate metabolic impairment (Ob.IR+, Ob.IR-; respectively) was found. Different biomarkers were analyzed after fasting (T = 0) and during an OGTT (T = 60 and 120 min). Lipoperoxides were measured in plasma, erythrocytes, and urine; while advanced glycation end products were determined in plasma, and redox status (GSH/GSSG ratio) in erythrocytes.ResultsWe found marked differences in the characterization of the oxidative status in urine and erythrocytes, and in the dynamics of the antioxidant response during OGTT. Specifically, Ob.IR+ children show increased oxidative stress, deficient antioxidant response and a significant imbalance in redox status, in   to controls and Ob.IR- children.ConclusionObese children with insulin resistance show increased levels of oxidative stress biomarkers, and a stunted antioxidant response to an OGTT leading to increased oxidative stress after a single glucose load, as detected in erythrocytes, but not in plasma. We propose erythrocyte as sensors of early and acute changes in oxidative stress associated with insulin resistance in childhood obesity. This is a pilot study, performed with a limited sample size, so data should be interpreted with caution until reproduced.
PB Ed. PUBLIC LIBRARY SCIENCE
SN 1932-6203
YR 2018
FD 2018-01-25
LK http://hdl.handle.net/10498/35071
UL http://hdl.handle.net/10498/35071
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 12-nov-2026