RT journal article
T1 Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia
A1 Blanco-Andújar, Cristina
A1 Ortega Ponce, Daniel
A1 Southern, Paul
A1 Steven, Nesbitt
A1 Thanh, Nguyen Thi Kim
A1 Pankhurst, Quentin Andrew
A2 Física de la Materia Condensada
K1 apoptosis
K1 magnetic hyperthermia
K1 in vitro
K1 melanoma
AB Aim: To assess cell death pathways in response to magnetic hyperthermia. Materials & methods: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. Results: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. Conclusion: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
SN 1748-6963
YR 2015
FD 2015-12-11
LK http://hdl.handle.net/10498/35264
UL http://hdl.handle.net/10498/35264
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 21-sep-2026