RT journal article
T1 Understanding the Biology of the Harmless Isolate Botrytis cinerea B459: An Approach to Bio-Targeted Toxin Identification
A1 Coca Ruiz, Víctor
A1 Cabrera Gómez, Nuria
A1 Saborido Torres, David
A1 Casado del Castillo, Virginia
A1 Benito, Ernesto P.
A1 Aleu Casatejada, Josefina
A1 González Collado, Isidro
A2 Química Orgánica
K1 B. cinerea
K1 phenotypic characterization
K1 secondary metabolism
K1 toxins
AB Botrytis cinerea, a necrotrophic fungus responsible for grey rot disease, causes substantial economic losses. However, recent studies have discovered distinct non-sporogenic and non-infective isolates of this species, such as the B459 field strain. Examination of these isolates is particularly intriguing in the context of the development of methodological applications that could be useful in the biocontrol of this phytopathogenic species. This investigation revealed that B459 exhibited a reduced growth rate yet displayed enhanced resilience to stressors like oxidative stress agents, SDS, ethanol, and PhITC. Notably, its ability to generate reactive oxygen species (ROS) and acidic compounds, crucial in plant–pathogen interactions, was impacted. Bio-targeted toxin identification assays and the metabolomic study of extracts obtained from fermentations at seven and fourteen days revealed that this strain does not biosynthesize botrydial and derivatives neither botcinin family toxins. Furthermore, its inability to infect tomato fruits, grape, and gerbera petals coincides with its lack of toxin production under culture conditions typically adapted for reference strain B05.10.
PB Multidisciplinary Digital Publishing Institute (MDPI)
SN 2077-0472
YR 2024
FD 2024
LK http://hdl.handle.net/10498/35462
UL http://hdl.handle.net/10498/35462
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 06-nov-2026