RT journal article
T1 Effect of bile salts on intestinal epithelial function in gilthead seabream (Sparus aurata)
A1 Fuentes, J.
A1 Filipa Gregório, Silvia
A1 Fonseca, F.
A1 Robles-Arozarena, R.
A1 Martos Sitcha, Juan Antonio
A1 Moyano, F.J.
A2 Biología
K1 Bile salts
K1 Intestine
K1 Sea bream
K1 Ussing chamber
AB In the context of modern aquaculture, the effort to reduce the reliance on fishmeal/marine ingredients in fish diets has led to the exploration of plant-based protein sources as potential substitutes, a dietary shift that disrupts the bile acid profile in fish. Therefore, bile salts are being sought as additives. However, artificially increased intestinal levels of bile acids may significantly impact mucosal function. Therefore, here, we explored the regulatory role in the intestine of gilthead sea bream (Sparus aurata) of (i) chenodeoxycholic acid (CDC), (ii) a mixture formed by two bile acids, 3% cholic acid and 97% deoxycholic acid (MIX), and (iii) a conjugated bile salt sodium taurocholate (TC) in Ussing chambers with the epithelial voltage clamp technique. We tested the bile salts in a 50–500 μg/ml concentration range, and all of them promoted ion absorption. Yet, clear concentration-dependent and more pronounced effects on the ion transport were observed in the posterior intestine. On the other hand, bile salts had no or minor effects on tissue resistance. However, there are indications that the MIX could have adverse effects at high concentrations (500 μg/ml), promoting a threefold increase in tissue permeability measured using FITC-dextran (4 kD) regardless of the intestinal region, thus suggesting an alteration in intestinal permeability at high bile salt concentrations. The findings from our study emphasize the importance of considering intestinal function when contemplating the possible use of a particular bile salt as a dietary supplement. It appears that bile salts, whether acting individually or in combination, play a pivotal role in orchestrating nutrient absorption by influencing the function of epithelial ion transport. However further research is needed to fully grasp the region-dependent nuances of bile salt effects on ion transport and the ultimate consequences for nutrient absorption in the context of fish aquaculture.
PB Springer Science and Business Media B.V.
SN 1573-5168
YR 2024
FD 2024
LK http://hdl.handle.net/10498/35514
UL http://hdl.handle.net/10498/35514
LA eng
DS Repositorio Institucional de la Universidad de Cádiz
RD 22-sep-2026